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egfp rab18q67l  (Addgene inc)


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    Structured Review

    Addgene inc egfp rab18q67l
    GDP‐bound Rab18 accumulates in perinuclear endosomes and promotes SHH‐XLEV secretion. (A–C) Airyscan microscopy of NIH3T3 cells stably expressing SHHN‐EGFP following treatment with ZSTK474 (1 µM, 1 h; A, upper), LY294002 (50 µM, 8 h; A, lower), or SF1670 (250 nM, overnight; B), with quantification of peripheral distribution scores (PDS; C). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 20. (D–F) Airyscan microscopy of NIH3T3 cells <t>expressing</t> <t>EGFP‐Rab18Q67L</t> (GTP‐bound mimetic; D) or EGFP‐Rab18S22N (GDP‐bound mimetic; E), with PDS quantification (F). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 7–16. (G–I) Airyscan immunofluorescence microscopy of EGFP‐Rab18S22N–expressing cells stained for AP‐1 (G) or AP‐3 (H) at low (upper) and high (lower) magnifications, with colocalization analysis (I). Arrowheads indicate perinuclear Rab18S22N puncta. Scale bars, 20 µm. *** p < 0.001; Welch's t test; n = 10.
    Egfp Rab18q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+rab18q67l/EGFP-Rab18+(Plasmid+%2349550)/pmc12848524-66-5-6
    Average 92 stars, based on 9 article reviews
    egfp rab18q67l - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Unconventional Secretion of Angiogenic Sonic Hedgehog–Containing Extra‐Large Extracellular Vesicles is Driven by PI3K–Rab18‐GDP Signalling"

    Article Title: Unconventional Secretion of Angiogenic Sonic Hedgehog–Containing Extra‐Large Extracellular Vesicles is Driven by PI3K–Rab18‐GDP Signalling

    Journal: Journal of Extracellular Biology

    doi: 10.1002/jex2.70112

    GDP‐bound Rab18 accumulates in perinuclear endosomes and promotes SHH‐XLEV secretion. (A–C) Airyscan microscopy of NIH3T3 cells stably expressing SHHN‐EGFP following treatment with ZSTK474 (1 µM, 1 h; A, upper), LY294002 (50 µM, 8 h; A, lower), or SF1670 (250 nM, overnight; B), with quantification of peripheral distribution scores (PDS; C). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 20. (D–F) Airyscan microscopy of NIH3T3 cells expressing EGFP‐Rab18Q67L (GTP‐bound mimetic; D) or EGFP‐Rab18S22N (GDP‐bound mimetic; E), with PDS quantification (F). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 7–16. (G–I) Airyscan immunofluorescence microscopy of EGFP‐Rab18S22N–expressing cells stained for AP‐1 (G) or AP‐3 (H) at low (upper) and high (lower) magnifications, with colocalization analysis (I). Arrowheads indicate perinuclear Rab18S22N puncta. Scale bars, 20 µm. *** p < 0.001; Welch's t test; n = 10.
    Figure Legend Snippet: GDP‐bound Rab18 accumulates in perinuclear endosomes and promotes SHH‐XLEV secretion. (A–C) Airyscan microscopy of NIH3T3 cells stably expressing SHHN‐EGFP following treatment with ZSTK474 (1 µM, 1 h; A, upper), LY294002 (50 µM, 8 h; A, lower), or SF1670 (250 nM, overnight; B), with quantification of peripheral distribution scores (PDS; C). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 20. (D–F) Airyscan microscopy of NIH3T3 cells expressing EGFP‐Rab18Q67L (GTP‐bound mimetic; D) or EGFP‐Rab18S22N (GDP‐bound mimetic; E), with PDS quantification (F). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 7–16. (G–I) Airyscan immunofluorescence microscopy of EGFP‐Rab18S22N–expressing cells stained for AP‐1 (G) or AP‐3 (H) at low (upper) and high (lower) magnifications, with colocalization analysis (I). Arrowheads indicate perinuclear Rab18S22N puncta. Scale bars, 20 µm. *** p < 0.001; Welch's t test; n = 10.

    Techniques Used: Microscopy, Stable Transfection, Expressing, Immunofluorescence, Staining

    Related Articles

    Plasmid Preparation:

    Article Title: Unconventional secretion of angiogenic sonic-hedgehog-containing large extracellular vesicles is stimulated via the PI3K–Rab18-GDP pathway
    Article Snippet: These expression units were transferred to the ViraPower Adenoviral Expression System (Thermo Fisher) according to the manufacturer’s instructions. .. EGFP-Rab18Q67L (Addgene plasmid #49596; https://www.addgene.org/49596 ; RRID: Addgene_49596), EGFP-Rab18S22N (Addgene plasmid #49597; https://www.addgene.org/49597 ; RRID: Addgene_49597), and EGFP-Rab18 (Addgene plasmid #49550; https://www.addgene.org/49550 ; RRID: Addgene_49550) expression vectors were gifts from Marci Scidmore (Huang et al., 2010). .. For expression of GST-tagged Rab18 proteins in Escherichia coli , the Rab18 fragment of each plasmid was amplified via PCR using the following primers: EcoRI+Rab18-F (5′-TCCGAATTCCATGGACGAGGACGTGCTGACCACTCTG-3′) and Not+Rab18-R (5′-AATGCGGCCGCCTATAGCACAGAGCAGTAACCGCCGCAGGCG-3′) and ligated with the pGEX-4T-3 vector (Cytiva Life Sciences) at EcoRI and NotI sites.

    Expressing:

    Article Title: Unconventional secretion of angiogenic sonic-hedgehog-containing large extracellular vesicles is stimulated via the PI3K–Rab18-GDP pathway
    Article Snippet: These expression units were transferred to the ViraPower Adenoviral Expression System (Thermo Fisher) according to the manufacturer’s instructions. .. EGFP-Rab18Q67L (Addgene plasmid #49596; https://www.addgene.org/49596 ; RRID: Addgene_49596), EGFP-Rab18S22N (Addgene plasmid #49597; https://www.addgene.org/49597 ; RRID: Addgene_49597), and EGFP-Rab18 (Addgene plasmid #49550; https://www.addgene.org/49550 ; RRID: Addgene_49550) expression vectors were gifts from Marci Scidmore (Huang et al., 2010). .. For expression of GST-tagged Rab18 proteins in Escherichia coli , the Rab18 fragment of each plasmid was amplified via PCR using the following primers: EcoRI+Rab18-F (5′-TCCGAATTCCATGGACGAGGACGTGCTGACCACTCTG-3′) and Not+Rab18-R (5′-AATGCGGCCGCCTATAGCACAGAGCAGTAACCGCCGCAGGCG-3′) and ligated with the pGEX-4T-3 vector (Cytiva Life Sciences) at EcoRI and NotI sites.



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    egfp rab18q67l  (Addgene inc)
    92
    Addgene inc egfp rab18q67l
    GDP‐bound Rab18 accumulates in perinuclear endosomes and promotes SHH‐XLEV secretion. (A–C) Airyscan microscopy of NIH3T3 cells stably expressing SHHN‐EGFP following treatment with ZSTK474 (1 µM, 1 h; A, upper), LY294002 (50 µM, 8 h; A, lower), or SF1670 (250 nM, overnight; B), with quantification of peripheral distribution scores (PDS; C). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 20. (D–F) Airyscan microscopy of NIH3T3 cells <t>expressing</t> <t>EGFP‐Rab18Q67L</t> (GTP‐bound mimetic; D) or EGFP‐Rab18S22N (GDP‐bound mimetic; E), with PDS quantification (F). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 7–16. (G–I) Airyscan immunofluorescence microscopy of EGFP‐Rab18S22N–expressing cells stained for AP‐1 (G) or AP‐3 (H) at low (upper) and high (lower) magnifications, with colocalization analysis (I). Arrowheads indicate perinuclear Rab18S22N puncta. Scale bars, 20 µm. *** p < 0.001; Welch's t test; n = 10.
    Egfp Rab18q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+rab18q67l/EGFP-Rab18+(Plasmid+%2349550)/pmc12848524-66-5-6
    Average 92 stars, based on 1 article reviews
    egfp rab18q67l - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    GDP‐bound Rab18 accumulates in perinuclear endosomes and promotes SHH‐XLEV secretion. (A–C) Airyscan microscopy of NIH3T3 cells stably expressing SHHN‐EGFP following treatment with ZSTK474 (1 µM, 1 h; A, upper), LY294002 (50 µM, 8 h; A, lower), or SF1670 (250 nM, overnight; B), with quantification of peripheral distribution scores (PDS; C). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 20. (D–F) Airyscan microscopy of NIH3T3 cells expressing EGFP‐Rab18Q67L (GTP‐bound mimetic; D) or EGFP‐Rab18S22N (GDP‐bound mimetic; E), with PDS quantification (F). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 7–16. (G–I) Airyscan immunofluorescence microscopy of EGFP‐Rab18S22N–expressing cells stained for AP‐1 (G) or AP‐3 (H) at low (upper) and high (lower) magnifications, with colocalization analysis (I). Arrowheads indicate perinuclear Rab18S22N puncta. Scale bars, 20 µm. *** p < 0.001; Welch's t test; n = 10.

    Journal: Journal of Extracellular Biology

    Article Title: Unconventional Secretion of Angiogenic Sonic Hedgehog–Containing Extra‐Large Extracellular Vesicles is Driven by PI3K–Rab18‐GDP Signalling

    doi: 10.1002/jex2.70112

    Figure Lengend Snippet: GDP‐bound Rab18 accumulates in perinuclear endosomes and promotes SHH‐XLEV secretion. (A–C) Airyscan microscopy of NIH3T3 cells stably expressing SHHN‐EGFP following treatment with ZSTK474 (1 µM, 1 h; A, upper), LY294002 (50 µM, 8 h; A, lower), or SF1670 (250 nM, overnight; B), with quantification of peripheral distribution scores (PDS; C). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 20. (D–F) Airyscan microscopy of NIH3T3 cells expressing EGFP‐Rab18Q67L (GTP‐bound mimetic; D) or EGFP‐Rab18S22N (GDP‐bound mimetic; E), with PDS quantification (F). Scale bars, 20 µm. ** p < 0.01; Welch's t test; n = 7–16. (G–I) Airyscan immunofluorescence microscopy of EGFP‐Rab18S22N–expressing cells stained for AP‐1 (G) or AP‐3 (H) at low (upper) and high (lower) magnifications, with colocalization analysis (I). Arrowheads indicate perinuclear Rab18S22N puncta. Scale bars, 20 µm. *** p < 0.001; Welch's t test; n = 10.

    Article Snippet: EGFP‐Rab18 (Addgene #49550; RRID: Addgene_49550), EGFP‐Rab18Q67L (Addgene #49596; RRID: Addgene_49596), and EGFP‐Rab18S22N (Addgene #49597; RRID: Addgene_49597) were gifts from Marci Scidmore (Huang et al. ).

    Techniques: Microscopy, Stable Transfection, Expressing, Immunofluorescence, Staining